Cuddles needed the treatment after he stopped eating late last year.
After his injection, the keepers coiled him into a crate for a 20-minute nebuliser treatment of inhalable anti-bacterial medication to help destroy any bacteria that may be causing him problems.
Perth Zoo chief vet Simone Vitali said Cuddles had been looking peaky for several weeks but the treatment regime was starting to show results and he had recently eaten a small chicken.
She said the four keepers were needed due to his strength.
‘Pythons are like one big ball of muscle,’ she said.
‘While they are not venomous, they can deliver a severe bite.’
Cuddles can be seen at Perth Zoo in the Reptile Encounter exhibit.