The city identified the lemon-scented gum as being poisoned in mid-May after it began displaying signs of chlorosis, a sudden loss of chlorophyll, symptomatic of herbicide poisoning.
The city valued the tree, on Edinboro Place, at nearly $17,000 under the Helliwell system.
It will seek this amount plus the costs of removing and replacing the tree after a successful investigation and subsequent prosecution.
Stirling acting parks and reserves manager Drew Manning said the city did not know who had poisoned the tree.
�The city placed a sign on the tree advising that the tree has been poisoned requesting for any information,� Mr Manning said.
�No residents have come forward as being a witness at this stage.�
The resident said poisoning a local tree was senseless and selfish.
�I think it�s a complete disregard for nature and what the tree provides for us,� the resident said.
�That tree is iconic on our street and it has been for 30 years.
�Now it might have to be chopped down because some idiot has done this.�
Anyone who has any possible information on the tree poisoning, they are asked to call the City of Stirling on 9205 8555.