Bob Bollen and TJDFC board member Janine Goyder paid $10,000 each for the Vidot painting, before donating the piece to the centre.
Centre general manager Rebecca Johnson said she was extremely grateful for the donation from Ms Goyder and Mr Bollen, who both have children with type 1 diabetes.
"We are not funded by government and we rely on community support to deliver our programs and services," Ms Johnson said.
"Community support has made the Family Centre vision a reality, and is critical to our sustainability and our impact."
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READ NOWMs Goyder said the centre provided an important hub for families to learn about type 1 diabetes and meet others in a similar situation to themselves,
"I think this might be the first of its kind in Australia, and it's really good for families as it can be quite lonely when kids are diagnosed with diabetes.
"Parents often don't have the support, so to be able to come here and get this education and support is fantastic," Ms Goyder said. The centre opened its doors earlier this year, connecting families of children with diabetes.